A physical altercation at a house party in Allston, just hours into the New Year, led to the stabbing death of a 21-year-old student, and the first reported homicide in the city in 2013.

On Wednesday, January 2, Brian MacDonald, 24, was in Brighton District Court facing charges for the murder of Anthony Spaulding.

MacDonald pleaded not guilty to the charges and was held on $500,000 bail.

According to officials, just before 3 a.m. on January 1, police found Spaulding unconscious in the snow outside of his Pratt Street apartment, suffering from stab wounds.

Witnesses said MacDonald and his girlfriend were at Spaulding’s house for a party when Spaulding had asked the pair to quiet down shortly after they arrived.

Officials said in court Wednesday that this “led to an almost immediate physical altercation between the two men,” with Spaulding and MacDonald tumbling down the stairs and then outside.

“The two men went outside, the evidence suggests, where they were the only two men at the scene. A bystander separated the two and observed blood on both of them,” according to Assistant District Attorney Holly Broadbent.

Witnesses allegedly told investigators that they had seen MacDonald with a knife at the party before Spaulding was fatally stabbed.

Investigators were able to follow a trail of blood from where the fight occurred all the way to MacDonald’s address, after learning his name.

When taken into custody by Boston Police, he had injuries to his face and hand. Surveillance imagery from a nearby camera also showed him fleeing the scene on foot, police said.

Spaulding succumbed to his stabbing injuries at Beth Israel Deaconess Medical Center shortly after the altercation in Allston.

According to his Facebook page, Spaulding was studying Audio Engineering at the New England Institute of Art in Brookline.

He was originally from Harwich, Massachusetts.

On Twitter, friends mourned the loss of the talented student.